DNA SEQUENZIERUNGNEXT GEN SEQUENCINGDNA/RNA OLIGOSFORSCHUNGLOGINSEQLAB

Premium Run Service

Are you looking for a personalized sequencing service that includes comprehensive customer support and troubleshooting? Are you looking for a top-quality sequencing service that is able to decode even difficult sequences? Then we recommend taking a closer look at our Premium Run Service.

 

To learn more about this type of sequencing service, please see the various sections below. To obtain information about prices, please enter our webshop and download the current price list.

 

Main Service Features and Benefits:

  • Read length per run: up to 1100 bases

  • Standard primers and specific primers will be added free of charge.

  • Rapid turnaround time: sequencing time down to <24h 1

  • Failed reactions will be repeated free of charge.

  • Convenient service: easy ordering and overnight in-house production of specific sequencing primers

  • Comprehensive support and troubleshooting via our experienced academic staff (no waiting on a recorded-message hotline):

    • Depending on your requirements, sequences can be delivered as edited or non-edited. Editing includes a manual proofread of your chromatogram by our experienced staff. Software-derived mistakes or uncertainties resulting from base calling (this is the procedure where the chromatogram is translated into your sequence) will be corrected.

    • Upon receiving your samples, we will check the DNA concentration; if DNA concentration is too low, we will approach you to discuss the further proceeding.

    • Depending on your requirements, results are provided via e-mail or via download from a HTTPS server. FASTA files are attached to the chromatograms.

    • Paper printouts are made available upon request.

    • In case of failed or poor sequencing results, we will approach you and make useful suggestions for issue solving (e.g. poly stretches, high G/C content, hairpin structures etc.). However, whether we continue with sequencing or stop at this stage is always your decision.

    • User-friendly and cost-free sample shipment: Just use either Seqlab-Microsynth’s pick-up service or request our pre-paid and pre-addressed envelopes for cost-free shipment via post mail.

  • Additional services:

    • One-Drop Sequencing

    • Special Treatment

    • Plasmid Preparation from E. coli

    • PCR Purification

Sample Requirements

General Information:

Each DNA sample and each primer must have a minimum volume of 20 µl. DNA samples and primers for sequencing reactions are best dissolved in pure water. Alternatively, 10 mM Tris-HCl (pH 8) or 10 mM Tris-HCl (pH 8) with a maximum of 0.01 mM EDTA can be used for a better long term DNA stability. Standard TE-buffer is not suitable, because higher EDTA concentrations inhibit sequencing polymerase activity. DNA samples will be stored for 3 months, whereas primers will be kept at Seqlab-Microsynth for 1 year.

 

Sample Amounts & Concentrations

DNA Template

Concentration

Effective Amount

 (in 20 µl)

For Each Additional Reaction

Plasmid

100 ng/µl

2 µg

+ 5 µl

PCR (<200bp)

5.0 ng/µl

0.1 µg

+ 5 µl

PCR (<500bp)

10 ng/µl

0.2 µg

+ 5 µl

PCR (<1000bp)

20 ng/µl

0.4 µg

+ 5 µl

PCR (<5000bp)

40 ng/µl

0.8 µg

+ 5 µl

PCR (>5000bp)

100 ng/µl

2 µg

+ 5 µl

Primer

10 pmol/µl = 10 µM

200 pmol

+ 5 µl

 

Important – Please Note!

Free Pick-up Service:

Our customers in Germany may benefit from our free pick-up service. In order to determine whether there is a Seqlab-Microsynth collection box in your immediate vicinity, please register/login and see under “View Collection Points”. For all customers without a collection box close by, Seqlab-Microsynth offers convenient and cost-free shipment of samples via its pre-paid and pre-addressed envelopes.

 

How to Order?  

  • Enter our webshop

  • Click on Select Services in the “DNA Sequencing” menu

  • Click on Order Form in the “Premium Run” domain and follow the further instructions

 


1Samples arriving between 9 AM and 11 AM are processed the same day and sequencing results are already available in the morning of the next working day. 

Seqlab - Sequence Laboratories Göttingen GmbH
Hannah-Vogt-Str. 1
37085 Göttingen

Postfach 3343
37023 Göttingen

Telefon 0551-370 00 10
Telefax 0551-370 00 12

E-Mail info@seqlab.de

Standard Primer List

Summarizes the pool of primers which are routinely stored at Seqlab-Microsynth and where you can select from (free of charge!).
Download list here.

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