Beyond plain DNA sequencing services, Seqlab-Microsynth offers a variety of useful additional services to make your life easier that are suitable for decoding even difficult-to-sequence regions/samples. In general you can select between the following four services:
To receive more information about these useful services, please see the various sections below. To obtain information about pricing, please enter our webshop and download the current price list. Please note that these services are only available in combination with our Premium Run Service or High Throughput Service.
Seqlab-Microsynth’s Special Treatment Service is especially useful if you want to decode difficult-to-sequence constructs such as siRNA or regions containing hairpin structures, G/C-rich sequences, etc.
Continuous optimization of our Sanger sequencing capabilities over the last 18 years has resulted in protocols that are able to map difficult or even very difficult DNA templates with a success rate >98%.
Generally, we recommend the following two-stage approach to our customers:
Seqlab-Microsynth’s One-Drop Sequencing Service is especially helpful if you want to sequence plasmid DNA but don’t have sufficient DNA. By applying this type of service, we are able to perform sequencing if you provide us with 50 ng or at least 1 µl of your DNA (one drop). It takes only one additional day. Therefore, the usual duration for entire sequencing of plasmid DNA will be 2 days.
Single Plasmid Preparation Service
You send us your E. coli cultures as single colonies grown on agar plates stating the antibiotic resistance. We pick your clones and amplify them before we isolate the plasmid DNA to be sequenced.
Please Note: E. coli cultures arriving at Seqlab-Microsynth before 12:00 AM will be picked the same day. Plasmid preparation takes only one day. Therefore, the first sequencing results can be expected usually after 2 working days.
High-Throughput Plasmid Preparation Service
Plasmid preparations at Seqlab-Microsynth will be performed in 96- or 384-well plates. For E. coli cells, we recommend sending them in 96- or 384-well plates as frozen glycerol stocks or in Luria Broth (LB) medium at room temperature (good sealing necessary!).
BAC, PAC, Fosmid, Cosmid DNA Preparation Service
You send us your E. coli cultures as single colonies grown on agar plates or as stab cultures stating the antibiotic resistance. We pick your clones and amplify them before we isolate the DNA templates to be sequenced.
Purifcation Service for Plasmids
Plasmid DNA is purified by ethanol precipitation.
Purifcation Service for PCR Products
PCR primers and leftover dNTPs are removed from your PCR reactions. Please be aware that this purification service does not separate individual PCR fragments!