Clone and Sequence
PCR amplification of heterogeneous biological samples results in a heterogeneous mixture of PCR products (e.g. ribosomal 16S PCR generated from stool or soil samples). In order to be able to sequence individual PCR products by DNA Sanger sequencing, it is first necessary to clone them. At Microsynth, we are able to perform the entire process of cloning and sequencing, no matter how many clones your sequencing project may require.
Beyond Sanger DNA sequencing, we are also able to offer you comprehensive next-generation sequencing services. In general, a next-generation sequencing approach allows to circumvent the cloning step and becomes more economical with increasing sample numbers and throughput requirements. Please contact our experts to figure out which approach fits best your needs.